Isolation of the B Cell Immune Synapse for Proteomic Analysis

: Cunha Diogo M, Hernández-Pérez Sara, Mattila Pieta K

: Baldari, C.T., Dustin, M.L.

: 2023

: Methods in Molecular Biology

: The Immune Synapse

: Methods in molecular biology (Clifton, N.J.)

: Methods Mol Biol

: Methods in Molecular Biology

: 2654

: 393

: 408

: 978-1-0716-3134-8

: 978-1-0716-3135-5

: 1064-3745

: 1940-6029

DOI: https://doi.org/10.1007/978-1-0716-3135-5_25

: https://research.utu.fi/converis/portal/detail/Publication/179763891

Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.

CunhaEtAl2023IsolationOfTheBCell.pdf