A3 Refereed book chapter or chapter in a compilation book

Isolation of the B Cell Immune Synapse for Proteomic Analysis



Authors: Cunha Diogo M, Hernández-Pérez Sara, Mattila Pieta K

Editors: Baldari, C.T., Dustin, M.L.

Publication year: 2023

Journal: Methods in Molecular Biology

Book title : The Immune Synapse

Journal name in source: Methods in molecular biology (Clifton, N.J.)

Journal acronym: Methods Mol Biol

Series title: Methods in Molecular Biology

Volume: 2654

First page : 393

Last page: 408

ISBN: 978-1-0716-3134-8

eISBN: 978-1-0716-3135-5

ISSN: 1064-3745

eISSN: 1940-6029

DOI: https://doi.org/10.1007/978-1-0716-3135-5_25

Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/179763891


Abstract
Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.

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Last updated on 2025-08-08 at 09:45