A3 Refereed book chapter or chapter in a compilation book
Isolation of the B Cell Immune Synapse for Proteomic Analysis
Authors: Cunha Diogo M, Hernández-Pérez Sara, Mattila Pieta K
Editors: Baldari, C.T., Dustin, M.L.
Publication year: 2023
Journal: Methods in Molecular Biology
Book title : The Immune Synapse
Journal name in source: Methods in molecular biology (Clifton, N.J.)
Journal acronym: Methods Mol Biol
Series title: Methods in Molecular Biology
Volume: 2654
First page : 393
Last page: 408
ISBN: 978-1-0716-3134-8
eISBN: 978-1-0716-3135-5
ISSN: 1064-3745
eISSN: 1940-6029
DOI: https://doi.org/10.1007/978-1-0716-3135-5_25
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/179763891
Abstract
Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.
Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.
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