A3 Vertaisarvioitu kirjan tai muun kokoomateoksen osa
Isolation of the B Cell Immune Synapse for Proteomic Analysis
Tekijät: Cunha Diogo M, Hernández-Pérez Sara, Mattila Pieta K
Toimittaja: Baldari, C.T., Dustin, M.L.
Julkaisuvuosi: 2023
Journal: Methods in Molecular Biology
Kokoomateoksen nimi: The Immune Synapse
Tietokannassa oleva lehden nimi: Methods in molecular biology (Clifton, N.J.)
Lehden akronyymi: Methods Mol Biol
Sarjan nimi: Methods in Molecular Biology
Vuosikerta: 2654
Aloitussivu: 393
Lopetussivu: 408
ISBN: 978-1-0716-3134-8
eISBN: 978-1-0716-3135-5
ISSN: 1064-3745
eISSN: 1940-6029
DOI: https://doi.org/10.1007/978-1-0716-3135-5_25
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/179763891
Tiivistelmä
Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.
Recent technical developments have fueled increasing utilization of proteomics to gain new insights into various aspects of cellular behavior. In this chapter, we describe a method to specifically isolate immune synapses from mouse primary B cells. The method utilizes antibody-coated magnetic beads to induce the formation of the immune synapses and describes a protocol for the extraction of the cell-bead adhesions for mass spectrometry analysis. Finally, this method enables unveiling the large-scale protein content of the immune synapse.
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