Expanding sample volume for microscopical detection of nanoplastics
: Hiltunen, Arto; Huopalainen, Joona; Mäkilä, Ermei; Häkkilä, Sirkku; Damlin, Pia; Hänninen, Jari
Publisher: Elsevier BV
: 2024
: Marine Environmental Research
: Marine Environmental Research
: 106806
: 202
: 0141-1136
: 1879-0291
DOI: https://doi.org/10.1016/j.marenvres.2024.106806
: https://doi.org/10.1016/j.marenvres.2024.106806
: https://research.utu.fi/converis/portal/detail/Publication/459062647
The extent of nanoplastic pollution has raised severe environmental and health concerns. While the means for microplastic detection are abundant, improved tools for nanoplastic detection are called-for. State-of-the-art microscopic techniques can detect nanoplastics down to tens of nanometers, however, only from small sample sizes (typically ∼ 10 μl). In this work, we describe a method that enables sampling of 1 l of seawater by the means of correlative Raman- and SEM-techniques. This is achieved by adapting common microplastic sample purification protocols to suit the nanoplastic study. In addition, we decorate a membrane filter with SERS-property to amplify the Raman signals. Together, the purification method combined with the use of the SERS-activated-membrane-filter enables identification and imaging of individual nanoplastic particles from significantly larger sample sizes than before. In the nanoscale the average recovery rate is 5 %. These results aim to provide useful tools for researchers in the fight against plastic pollution.
:
Sakari Alhopuro Foundation