Luminescence-Based Techniques for KRAS Thermal Stability Monitoring

: Kopra Kari

: Stephen Andrew G., Esposito Dominic

: New York

: 2024

: Methods in Molecular Biology

: KRAS : Methods and Protocols

: Methods in molecular biology (Clifton, N.J.)

: Methods Mol Biol

: Methods in Molecular Biology

: 2797

: 125

: 143

: 978-1-0716-3821-7

: 978-1-0716-3822-4

: 1064-3745

: 1940-6029

DOI: https://doi.org/10.1007/978-1-0716-3822-4_10

: https://link.springer.com/protocol/10.1007/978-1-0716-3822-4_10

Various biochemical methods have been introduced to detect and characterize KRAS activity and interactions, from which the vast majority is based on luminescence detection in its varying forms. Among these methods, thermal stability assays, using luminophore-conjugated proteins or external environment sensing dyes, are widely used. In this chapter, we describe methods enabling KRAS stability monitoring in vitro, with an emphasis on ligand-induced stability. This chapter focuses mainly on luminescence-based techniques utilizing external dye molecules and fluorescence detection.