A3 Vertaisarvioitu kirjan tai muun kokoomateoksen osa
Luminescence-Based Techniques for KRAS Thermal Stability Monitoring
Tekijät: Kopra Kari
Toimittaja: Stephen Andrew G., Esposito Dominic
Kustannuspaikka: New York
Julkaisuvuosi: 2024
Journal: Methods in Molecular Biology
Kokoomateoksen nimi: KRAS : Methods and Protocols
Tietokannassa oleva lehden nimi: Methods in molecular biology (Clifton, N.J.)
Lehden akronyymi: Methods Mol Biol
Sarjan nimi: Methods in Molecular Biology
Vuosikerta: 2797
Aloitussivu: 125
Lopetussivu: 143
ISBN: 978-1-0716-3821-7
eISBN: 978-1-0716-3822-4
ISSN: 1064-3745
eISSN: 1940-6029
DOI: https://doi.org/10.1007/978-1-0716-3822-4_10
Verkko-osoite: https://link.springer.com/protocol/10.1007/978-1-0716-3822-4_10
Tiivistelmä
Various biochemical methods have been introduced to detect and characterize KRAS activity and interactions, from which the vast majority is based on luminescence detection in its varying forms. Among these methods, thermal stability assays, using luminophore-conjugated proteins or external environment sensing dyes, are widely used. In this chapter, we describe methods enabling KRAS stability monitoring in vitro, with an emphasis on ligand-induced stability. This chapter focuses mainly on luminescence-based techniques utilizing external dye molecules and fluorescence detection.
Various biochemical methods have been introduced to detect and characterize KRAS activity and interactions, from which the vast majority is based on luminescence detection in its varying forms. Among these methods, thermal stability assays, using luminophore-conjugated proteins or external environment sensing dyes, are widely used. In this chapter, we describe methods enabling KRAS stability monitoring in vitro, with an emphasis on ligand-induced stability. This chapter focuses mainly on luminescence-based techniques utilizing external dye molecules and fluorescence detection.
Turun yliopiston Tutkimustietojärjestelmän portaali