A3 Refereed book chapter or chapter in a compilation book

Luminescence-Based Techniques for KRAS Thermal Stability Monitoring




AuthorsKopra Kari

EditorsStephen Andrew G., Esposito Dominic

Publishing placeNew York

Publication year2024

JournalMethods in Molecular Biology

Book title KRAS : Methods and Protocols

Journal name in sourceMethods in molecular biology (Clifton, N.J.)

Journal acronymMethods Mol Biol

Series titleMethods in Molecular Biology

Volume2797

First page 125

Last page143

ISBN978-1-0716-3821-7

eISBN978-1-0716-3822-4

ISSN1064-3745

eISSN1940-6029

DOIhttps://doi.org/10.1007/978-1-0716-3822-4_10

Web address https://link.springer.com/protocol/10.1007/978-1-0716-3822-4_10


Abstract
Various biochemical methods have been introduced to detect and characterize KRAS activity and interactions, from which the vast majority is based on luminescence detection in its varying forms. Among these methods, thermal stability assays, using luminophore-conjugated proteins or external environment sensing dyes, are widely used. In this chapter, we describe methods enabling KRAS stability monitoring in vitro, with an emphasis on ligand-induced stability. This chapter focuses mainly on luminescence-based techniques utilizing external dye molecules and fluorescence detection.



Last updated on 2024-26-11 at 15:21