A3 Refereed book chapter or chapter in a compilation book
Luminescence-Based Techniques for KRAS Thermal Stability Monitoring
Authors: Kopra Kari
Editors: Stephen Andrew G., Esposito Dominic
Publishing place: New York
Publication year: 2024
Journal: Methods in Molecular Biology
Book title : KRAS : Methods and Protocols
Journal name in source: Methods in molecular biology (Clifton, N.J.)
Journal acronym: Methods Mol Biol
Series title: Methods in Molecular Biology
Volume: 2797
First page : 125
Last page: 143
ISBN: 978-1-0716-3821-7
eISBN: 978-1-0716-3822-4
ISSN: 1064-3745
eISSN: 1940-6029
DOI: https://doi.org/10.1007/978-1-0716-3822-4_10
Web address : https://link.springer.com/protocol/10.1007/978-1-0716-3822-4_10
Abstract
Various biochemical methods have been introduced to detect and characterize KRAS activity and interactions, from which the vast majority is based on luminescence detection in its varying forms. Among these methods, thermal stability assays, using luminophore-conjugated proteins or external environment sensing dyes, are widely used. In this chapter, we describe methods enabling KRAS stability monitoring in vitro, with an emphasis on ligand-induced stability. This chapter focuses mainly on luminescence-based techniques utilizing external dye molecules and fluorescence detection.
Various biochemical methods have been introduced to detect and characterize KRAS activity and interactions, from which the vast majority is based on luminescence detection in its varying forms. Among these methods, thermal stability assays, using luminophore-conjugated proteins or external environment sensing dyes, are widely used. In this chapter, we describe methods enabling KRAS stability monitoring in vitro, with an emphasis on ligand-induced stability. This chapter focuses mainly on luminescence-based techniques utilizing external dye molecules and fluorescence detection.
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