Kinetics of photo-processes in dye molecules and the resolution of two-photon excitation fluorescence microscope with nanosecond laser



: Kleymionov E., Ivanov V., Meltola N., Hanninen P., Soini E.

: Privalov V.E.

: 2001

: Proceedings of SPIE - The International Society for Optical Engineering

: 4316

: 6

: 12

: 7

: 0-8194-3997-5

: 0277-786X

DOI: https://doi.org/10.1117/12.407664

: http://api.elsevier.com/content/abstract/scopus_id:0035046464


The dependence of the axial resolution of two-photon excitation (TPE) fluorescence microscope with nanosecond pulse laser on the kinetics of photoprocesses in dye molecules has been studied. It is shown that saturation effects and the fluorescence quenching due to absorption of the third photon by the excited dye molecule can deteriorate substantially the microscope resolution.



Last updated on 13/10/2025 02:20:20 PM