A4 Vertaisarvioitu artikkeli konferenssijulkaisussa
Kinetics of photo-processes in dye molecules and the resolution of two-photon excitation fluorescence microscope with nanosecond laser
Tekijät: Kleymionov E., Ivanov V., Meltola N., Hanninen P., Soini E.
Toimittaja: Privalov V.E.
Julkaisuvuosi: 2001
Tietokannassa oleva lehden nimi: Proceedings of SPIE - The International Society for Optical Engineering
Vuosikerta: 4316
Aloitussivu: 6
Lopetussivu: 12
Sivujen määrä: 7
ISBN: 0-8194-3997-5
ISSN: 0277-786X
DOI: https://doi.org/10.1117/12.407664
Verkko-osoite: http://api.elsevier.com/content/abstract/scopus_id:0035046464
Tiivistelmä
The dependence of the axial resolution of two-photon excitation (TPE) fluorescence microscope with nanosecond pulse laser on the kinetics of photoprocesses in dye molecules has been studied. It is shown that saturation effects and the fluorescence quenching due to absorption of the third photon by the excited dye molecule can deteriorate substantially the microscope resolution.
The dependence of the axial resolution of two-photon excitation (TPE) fluorescence microscope with nanosecond pulse laser on the kinetics of photoprocesses in dye molecules has been studied. It is shown that saturation effects and the fluorescence quenching due to absorption of the third photon by the excited dye molecule can deteriorate substantially the microscope resolution.
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