A4 Refereed article in a conference publication
Kinetics of photo-processes in dye molecules and the resolution of two-photon excitation fluorescence microscope with nanosecond laser
Authors: Kleymionov E., Ivanov V., Meltola N., Hanninen P., Soini E.
Editors: Privalov V.E.
Publication year: 2001
Journal name in source: Proceedings of SPIE - The International Society for Optical Engineering
Volume: 4316
First page : 6
Last page: 12
Number of pages: 7
ISBN: 0-8194-3997-5
ISSN: 0277-786X
DOI: https://doi.org/10.1117/12.407664
Web address : http://api.elsevier.com/content/abstract/scopus_id:0035046464
Abstract
The dependence of the axial resolution of two-photon excitation (TPE) fluorescence microscope with nanosecond pulse laser on the kinetics of photoprocesses in dye molecules has been studied. It is shown that saturation effects and the fluorescence quenching due to absorption of the third photon by the excited dye molecule can deteriorate substantially the microscope resolution.
The dependence of the axial resolution of two-photon excitation (TPE) fluorescence microscope with nanosecond pulse laser on the kinetics of photoprocesses in dye molecules has been studied. It is shown that saturation effects and the fluorescence quenching due to absorption of the third photon by the excited dye molecule can deteriorate substantially the microscope resolution.
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