Resolving fluorescence beads at 100-200 nm axial distance with a two-photon 4Pi-microscope operating in the near infrared



: Hell S., Schrader M., Hänninen P., Soini E.

: 1995

 Optics Communications

: Optics Communications

: 120

: null

: 129

: 133

: 5

: 0030-4018

: http://api.elsevier.com/content/abstract/scopus_id:0029388396


We show the possibility of resolving point objects having an axial distance of 100-200 nm with a precision of ± 15 nm with a two-photon excitation 4Pi-microscope. The resolution is demonstrated by axial imaging of clustered fluorescence beads. A comparison of the axial resolution of the two-photon excitation 4Pi-microscope with a regular two-photon excitation microscope shows a four-fold improvement in axial resolution. © 1995.



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