A1 Refereed original research article in a scientific journal

Resolving fluorescence beads at 100-200 nm axial distance with a two-photon 4Pi-microscope operating in the near infrared



Authors: Hell S., Schrader M., Hänninen P., Soini E.

Publication year: 1995

Journal:: Optics Communications

Journal name in source: Optics Communications

Volume: 120

Issue: null

First page : 129

Last page: 133

Number of pages: 5

ISSN: 0030-4018

Web address : http://api.elsevier.com/content/abstract/scopus_id:0029388396


Abstract
We show the possibility of resolving point objects having an axial distance of 100-200 nm with a precision of ± 15 nm with a two-photon excitation 4Pi-microscope. The resolution is demonstrated by axial imaging of clustered fluorescence beads. A comparison of the axial resolution of the two-photon excitation 4Pi-microscope with a regular two-photon excitation microscope shows a four-fold improvement in axial resolution. © 1995.



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