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Resolving fluorescence beads at 100-200 nm axial distance with a two-photon 4Pi-microscope operating in the near infrared
Tekijät: Hell S., Schrader M., Hänninen P., Soini E.
Julkaisuvuosi: 1995
Lehti:: Optics Communications
Tietokannassa oleva lehden nimi: Optics Communications
Vuosikerta: 120
Numero: null
Aloitussivu: 129
Lopetussivu: 133
Sivujen määrä: 5
ISSN: 0030-4018
Verkko-osoite: http://api.elsevier.com/content/abstract/scopus_id:0029388396
Tiivistelmä
We show the possibility of resolving point objects having an axial distance of 100-200 nm with a precision of ± 15 nm with a two-photon excitation 4Pi-microscope. The resolution is demonstrated by axial imaging of clustered fluorescence beads. A comparison of the axial resolution of the two-photon excitation 4Pi-microscope with a regular two-photon excitation microscope shows a four-fold improvement in axial resolution. © 1995.
We show the possibility of resolving point objects having an axial distance of 100-200 nm with a precision of ± 15 nm with a two-photon excitation 4Pi-microscope. The resolution is demonstrated by axial imaging of clustered fluorescence beads. A comparison of the axial resolution of the two-photon excitation 4Pi-microscope with a regular two-photon excitation microscope shows a four-fold improvement in axial resolution. © 1995.
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