Reaction kinetics of a two-photon excitation microparticle based immunoassay - From modelling to practice

: Hänninen P., Waris M., Kettunen M., Soini E.

: 2003

Biophysical Chemistry

: Biophysical Chemistry

: 105

: 1

: 23

: 28

: 6

: 0301-4622

DOI: https://doi.org/10.1016/S0301-4622(03)00042-5

: http://api.elsevier.com/content/abstract/scopus_id:0042661053

Real time observation of reaction kinetics is one of the key features of the newly developed microparticle based two-photon excitation fluorescence immunoassay system (TPX). By observing binding reactions at the surface of individual microparticles during the incubation of an assay, the binding constants of an assay become apparent. This paper describes the use of the new system in quantifying the reaction parameters of human thyroid stimulating hormone (hTSH) assay. A mechanistic reaction model for the assay is presented. The reaction model is further shown to precisely predict the behaviour of the assay kinetics over a wide range of analyte concentrations. © 2003 Elsevier B.V. All rights reserved.