A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Reaction kinetics of a two-photon excitation microparticle based immunoassay - From modelling to practice
Tekijät: Hänninen P., Waris M., Kettunen M., Soini E.
Julkaisuvuosi: 2003
Lehti:: Biophysical Chemistry
Tietokannassa oleva lehden nimi: Biophysical Chemistry
Vuosikerta: 105
Numero: 1
Aloitussivu: 23
Lopetussivu: 28
Sivujen määrä: 6
ISSN: 0301-4622
DOI: https://doi.org/10.1016/S0301-4622(03)00042-5
Verkko-osoite: http://api.elsevier.com/content/abstract/scopus_id:0042661053
Tiivistelmä
Real time observation of reaction kinetics is one of the key features of the newly developed microparticle based two-photon excitation fluorescence immunoassay system (TPX). By observing binding reactions at the surface of individual microparticles during the incubation of an assay, the binding constants of an assay become apparent. This paper describes the use of the new system in quantifying the reaction parameters of human thyroid stimulating hormone (hTSH) assay. A mechanistic reaction model for the assay is presented. The reaction model is further shown to precisely predict the behaviour of the assay kinetics over a wide range of analyte concentrations. © 2003 Elsevier B.V. All rights reserved.
Real time observation of reaction kinetics is one of the key features of the newly developed microparticle based two-photon excitation fluorescence immunoassay system (TPX). By observing binding reactions at the surface of individual microparticles during the incubation of an assay, the binding constants of an assay become apparent. This paper describes the use of the new system in quantifying the reaction parameters of human thyroid stimulating hormone (hTSH) assay. A mechanistic reaction model for the assay is presented. The reaction model is further shown to precisely predict the behaviour of the assay kinetics over a wide range of analyte concentrations. © 2003 Elsevier B.V. All rights reserved.
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