A1 Refereed original research article in a scientific journal
Reaction kinetics of a two-photon excitation microparticle based immunoassay - From modelling to practice
Authors: Hänninen P., Waris M., Kettunen M., Soini E.
Publication year: 2003
Journal:: Biophysical Chemistry
Journal name in source: Biophysical Chemistry
Volume: 105
Issue: 1
First page : 23
Last page: 28
Number of pages: 6
ISSN: 0301-4622
DOI: https://doi.org/10.1016/S0301-4622(03)00042-5
Web address : http://api.elsevier.com/content/abstract/scopus_id:0042661053
Abstract
Real time observation of reaction kinetics is one of the key features of the newly developed microparticle based two-photon excitation fluorescence immunoassay system (TPX). By observing binding reactions at the surface of individual microparticles during the incubation of an assay, the binding constants of an assay become apparent. This paper describes the use of the new system in quantifying the reaction parameters of human thyroid stimulating hormone (hTSH) assay. A mechanistic reaction model for the assay is presented. The reaction model is further shown to precisely predict the behaviour of the assay kinetics over a wide range of analyte concentrations. © 2003 Elsevier B.V. All rights reserved.
Real time observation of reaction kinetics is one of the key features of the newly developed microparticle based two-photon excitation fluorescence immunoassay system (TPX). By observing binding reactions at the surface of individual microparticles during the incubation of an assay, the binding constants of an assay become apparent. This paper describes the use of the new system in quantifying the reaction parameters of human thyroid stimulating hormone (hTSH) assay. A mechanistic reaction model for the assay is presented. The reaction model is further shown to precisely predict the behaviour of the assay kinetics over a wide range of analyte concentrations. © 2003 Elsevier B.V. All rights reserved.
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