Two-photon excitation 4Pi confocal microscope: Enhanced axial resolution microscope for biological research

: Hänninen P., Hell S., Salo J., Soini E., Cremer C.

: 1995

Applied Physics Letters

: Applied Physics Letters

: null

: 1698

: null

: 0003-6951

: http://api.elsevier.com/content/abstract/scopus_id:0029273602

The applicability of two photon excitation 4Pi confocal fluorescence microscopy to biological imaging is demonstrated. We show that 4Pi confocal microscopy in combination with a simple deconvolution algorithm allows axial localization and quantification with 0.14 μm resolution in a biological sample. The 4Pi-confocal microscope extends the applicability of far field fluorescence microscopy to high resolution three-dimensional imaging and quantification of subcellular structures.