A1 Refereed original research article in a scientific journal
Two-photon excitation 4Pi confocal microscope: Enhanced axial resolution microscope for biological research
Authors: Hänninen P., Hell S., Salo J., Soini E., Cremer C.
Publication year: 1995
Journal:: Applied Physics Letters
Journal name in source: Applied Physics Letters
Volume: null
Issue: null
First page : 1698
Last page: null
ISSN: 0003-6951
Web address : http://api.elsevier.com/content/abstract/scopus_id:0029273602
Abstract
The applicability of two photon excitation 4Pi confocal fluorescence microscopy to biological imaging is demonstrated. We show that 4Pi confocal microscopy in combination with a simple deconvolution algorithm allows axial localization and quantification with 0.14 μm resolution in a biological sample. The 4Pi-confocal microscope extends the applicability of far field fluorescence microscopy to high resolution three-dimensional imaging and quantification of subcellular structures.
The applicability of two photon excitation 4Pi confocal fluorescence microscopy to biological imaging is demonstrated. We show that 4Pi confocal microscopy in combination with a simple deconvolution algorithm allows axial localization and quantification with 0.14 μm resolution in a biological sample. The 4Pi-confocal microscope extends the applicability of far field fluorescence microscopy to high resolution three-dimensional imaging and quantification of subcellular structures.
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