A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Two-photon excitation 4Pi confocal microscope: Enhanced axial resolution microscope for biological research
Tekijät: Hänninen P., Hell S., Salo J., Soini E., Cremer C.
Julkaisuvuosi: 1995
Lehti:: Applied Physics Letters
Tietokannassa oleva lehden nimi: Applied Physics Letters
Vuosikerta: null
Numero: null
Aloitussivu: 1698
Lopetussivu: null
ISSN: 0003-6951
Verkko-osoite: http://api.elsevier.com/content/abstract/scopus_id:0029273602
Tiivistelmä
The applicability of two photon excitation 4Pi confocal fluorescence microscopy to biological imaging is demonstrated. We show that 4Pi confocal microscopy in combination with a simple deconvolution algorithm allows axial localization and quantification with 0.14 μm resolution in a biological sample. The 4Pi-confocal microscope extends the applicability of far field fluorescence microscopy to high resolution three-dimensional imaging and quantification of subcellular structures.
The applicability of two photon excitation 4Pi confocal fluorescence microscopy to biological imaging is demonstrated. We show that 4Pi confocal microscopy in combination with a simple deconvolution algorithm allows axial localization and quantification with 0.14 μm resolution in a biological sample. The 4Pi-confocal microscope extends the applicability of far field fluorescence microscopy to high resolution three-dimensional imaging and quantification of subcellular structures.
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