Annular aperture two-photon excitation microscopy



: Hell S., Hänninen P., Kuusisto A., Schrader M., Soini E.

: 1995

 Optics Communications

: Optics Communications

: 117

: null

: 20

: 24

: 0030-4018

: http://api.elsevier.com/content/abstract/scopus_id:58149363392


We investigate the effect of annular apertures in two-photon excitation confocal and non-confocal microscopy. In a theoretical study, we show that the use of annular apertures in two-photon excitation microscopy leads to resolution increase without diffraction fringes. We determine experimentally the axial resolution of annular aperture confocal and non-confocal two-photon excitation microscopes and compare it with that of the standard two-photon excitation contrasts. Images of fluorescence beads obtained with a transmission annular aperture two-photon excitation microscope demonstrate the applicability of annular apertures for enlarging the focal depth in high resolution two-photon excitation imaging. © 1995.



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