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Annular aperture two-photon excitation microscopy
Tekijät: Hell S., Hänninen P., Kuusisto A., Schrader M., Soini E.
Julkaisuvuosi: 1995
Lehti:: Optics Communications
Tietokannassa oleva lehden nimi: Optics Communications
Vuosikerta: 117
Numero: null
Aloitussivu: 20
Lopetussivu: 24
ISSN: 0030-4018
Verkko-osoite: http://api.elsevier.com/content/abstract/scopus_id:58149363392
Tiivistelmä
We investigate the effect of annular apertures in two-photon excitation confocal and non-confocal microscopy. In a theoretical study, we show that the use of annular apertures in two-photon excitation microscopy leads to resolution increase without diffraction fringes. We determine experimentally the axial resolution of annular aperture confocal and non-confocal two-photon excitation microscopes and compare it with that of the standard two-photon excitation contrasts. Images of fluorescence beads obtained with a transmission annular aperture two-photon excitation microscope demonstrate the applicability of annular apertures for enlarging the focal depth in high resolution two-photon excitation imaging. © 1995.
We investigate the effect of annular apertures in two-photon excitation confocal and non-confocal microscopy. In a theoretical study, we show that the use of annular apertures in two-photon excitation microscopy leads to resolution increase without diffraction fringes. We determine experimentally the axial resolution of annular aperture confocal and non-confocal two-photon excitation microscopes and compare it with that of the standard two-photon excitation contrasts. Images of fluorescence beads obtained with a transmission annular aperture two-photon excitation microscope demonstrate the applicability of annular apertures for enlarging the focal depth in high resolution two-photon excitation imaging. © 1995.
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