A1 Refereed original research article in a scientific journal
Annular aperture two-photon excitation microscopy
Authors: Hell S., Hänninen P., Kuusisto A., Schrader M., Soini E.
Publication year: 1995
Journal:: Optics Communications
Journal name in source: Optics Communications
Volume: 117
Issue: null
First page : 20
Last page: 24
ISSN: 0030-4018
Web address : http://api.elsevier.com/content/abstract/scopus_id:58149363392
Abstract
We investigate the effect of annular apertures in two-photon excitation confocal and non-confocal microscopy. In a theoretical study, we show that the use of annular apertures in two-photon excitation microscopy leads to resolution increase without diffraction fringes. We determine experimentally the axial resolution of annular aperture confocal and non-confocal two-photon excitation microscopes and compare it with that of the standard two-photon excitation contrasts. Images of fluorescence beads obtained with a transmission annular aperture two-photon excitation microscope demonstrate the applicability of annular apertures for enlarging the focal depth in high resolution two-photon excitation imaging. © 1995.
We investigate the effect of annular apertures in two-photon excitation confocal and non-confocal microscopy. In a theoretical study, we show that the use of annular apertures in two-photon excitation microscopy leads to resolution increase without diffraction fringes. We determine experimentally the axial resolution of annular aperture confocal and non-confocal two-photon excitation microscopes and compare it with that of the standard two-photon excitation contrasts. Images of fluorescence beads obtained with a transmission annular aperture two-photon excitation microscope demonstrate the applicability of annular apertures for enlarging the focal depth in high resolution two-photon excitation imaging. © 1995.
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