Two- and multiphoton excitation of conjugate-dyes using a continuous wave laser

: Hänninen P., Lehtelä L., Hell S.

: 1996

: Optics Communications

: 130

: null

: 29

: 33

: 5

: 0030-4018

: http://api.elsevier.com/content/abstract/scopus_id:0030241190

We discuss the possibility of obtaining a fluorescence signal depending nonlinearly on the excitation intensity by inhibiting the fluorescence energy transfer in a conjugate-pair dye molecule and its application in scanning microscopy. The dynamical behaviour of the conjugate dye molecule and the nonlinear behaviour of the fluorescence intensity is analysed. The benefits for scanning fluorescence microscopy are outlined.