A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Two- and multiphoton excitation of conjugate-dyes using a continuous wave laser
Tekijät: Hänninen P., Lehtelä L., Hell S.
Julkaisuvuosi: 1996
Lehti:: Optics Communications
Tietokannassa oleva lehden nimi: Optics Communications
Vuosikerta: 130
Numero: null
Aloitussivu: 29
Lopetussivu: 33
Sivujen määrä: 5
ISSN: 0030-4018
Verkko-osoite: http://api.elsevier.com/content/abstract/scopus_id:0030241190
Tiivistelmä
We discuss the possibility of obtaining a fluorescence signal depending nonlinearly on the excitation intensity by inhibiting the fluorescence energy transfer in a conjugate-pair dye molecule and its application in scanning microscopy. The dynamical behaviour of the conjugate dye molecule and the nonlinear behaviour of the fluorescence intensity is analysed. The benefits for scanning fluorescence microscopy are outlined.
We discuss the possibility of obtaining a fluorescence signal depending nonlinearly on the excitation intensity by inhibiting the fluorescence energy transfer in a conjugate-pair dye molecule and its application in scanning microscopy. The dynamical behaviour of the conjugate dye molecule and the nonlinear behaviour of the fluorescence intensity is analysed. The benefits for scanning fluorescence microscopy are outlined.
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