A1 Refereed original research article in a scientific journal

Two- and multiphoton excitation of conjugate-dyes using a continuous wave laser




AuthorsHänninen P., Lehtelä L., Hell S.

Publication year1996

Journal:Optics Communications

Journal name in sourceOptics Communications

Volume130

Issuenull

First page 29

Last page33

Number of pages5

ISSN0030-4018

Web address http://api.elsevier.com/content/abstract/scopus_id:0030241190


Abstract
We discuss the possibility of obtaining a fluorescence signal depending nonlinearly on the excitation intensity by inhibiting the fluorescence energy transfer in a conjugate-pair dye molecule and its application in scanning microscopy. The dynamical behaviour of the conjugate dye molecule and the nonlinear behaviour of the fluorescence intensity is analysed. The benefits for scanning fluorescence microscopy are outlined.



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