A1 Refereed original research article in a scientific journal
Two- and multiphoton excitation of conjugate-dyes using a continuous wave laser
Authors: Hänninen P., Lehtelä L., Hell S.
Publication year: 1996
Journal:: Optics Communications
Journal name in source: Optics Communications
Volume: 130
Issue: null
First page : 29
Last page: 33
Number of pages: 5
ISSN: 0030-4018
Web address : http://api.elsevier.com/content/abstract/scopus_id:0030241190
Abstract
We discuss the possibility of obtaining a fluorescence signal depending nonlinearly on the excitation intensity by inhibiting the fluorescence energy transfer in a conjugate-pair dye molecule and its application in scanning microscopy. The dynamical behaviour of the conjugate dye molecule and the nonlinear behaviour of the fluorescence intensity is analysed. The benefits for scanning fluorescence microscopy are outlined.
We discuss the possibility of obtaining a fluorescence signal depending nonlinearly on the excitation intensity by inhibiting the fluorescence energy transfer in a conjugate-pair dye molecule and its application in scanning microscopy. The dynamical behaviour of the conjugate dye molecule and the nonlinear behaviour of the fluorescence intensity is analysed. The benefits for scanning fluorescence microscopy are outlined.