Microparticle - based platform for point-of-care immunoassays



: Teppo Salminen, Etvi Juntunen, Merja Lahdenranta, Iida Martiskainen, Sheikh M. Talha, Kim Pettersson

Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE

: 2018

: Analytical Biochemistry

: ANALYTICAL BIOCHEMISTRY

: ANAL BIOCHEM

: 548

: 66

: 68

: 3

: 0003-2697

: 1096-0309

DOI: https://doi.org/10.1016/j.ab.2018.02.021

: https://research.utu.fi/converis/portal/detail/Publication/31197303


There is a need for quantitative and sensitive, yet simple point-of-care immunoassays: We have developed a point-of-care microparticle-based immunoassay platform which combines the performance of a microliter well based assay with the usability of a rapid assay. The platform contained a separate reaction and detection chambers and microparticles for the solid-phase. Photoluminescent up-converting nanoparticles (UCNPs) were used as labels. The platform was tested with a cardiac troponin I assay, and a limit of detection of 19.7 ng/L was obtained. This study demonstrates the feasibility of developing point-of-care assays on the new platform for various analytes of interests.

Last updated on 2024-26-11 at 11:24