Synthesis of Aminoglycoside-2 '-O-Methyl Oligoribonucleotide Fusions

: Granqvist L, Kraszewski A, Tähtinen V, Virta P

Publisher: MDPI AG

: Basel

: 2017

: Molecules

: MOLECULES

: ARTN 760

: 22

: 5

: 13

: 1420-3049

DOI: https://doi.org/10.3390/molecules22050760

: http://www.mdpi.com/1420-3049/22/5/760

: https://research.utu.fi/converis/portal/detail/Publication/25773013

Phosphoramidite building blocks of ribostamycin (3 and 4), that may be incorporated at any position of the oligonucleotide sequence, were synthesized. The building blocks, together with a previously described neomycin-modified solid support, were applied for the preparation of aminoglycoside-2'-O-methyl oligoribonucleotide fusions. The fusions were used to clamp a single strand DNA sequence (a purine-rich strand of c-Myc promoter 1) to form triple helical 2'-O-methyl RNA/DNA-hybrid constructs. The potential of the aminoglycoside moieties to stabilize the triple helical constructs were studied by UV-melting profile analysis.

molecules-22-00760.pdf