A1 Refereed original research article in a scientific journal
Synthesis of Aminoglycoside-2 '-O-Methyl Oligoribonucleotide Fusions
Authors: Granqvist L, Kraszewski A, Tähtinen V, Virta P
Publisher: MDPI AG
Publishing place: Basel
Publication year: 2017
Journal: Molecules
Journal name in source: MOLECULES
Journal acronym: MOLECULES
Article number: ARTN 760
Volume: 22
Issue: 5
Number of pages: 13
ISSN: 1420-3049
DOI: https://doi.org/10.3390/molecules22050760
Web address : http://www.mdpi.com/1420-3049/22/5/760
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/25773013
Phosphoramidite building blocks of ribostamycin (3 and 4), that may be incorporated at any position of the oligonucleotide sequence, were synthesized. The building blocks, together with a previously described neomycin-modified solid support, were applied for the preparation of aminoglycoside-2'-O-methyl oligoribonucleotide fusions. The fusions were used to clamp a single strand DNA sequence (a purine-rich strand of c-Myc promoter 1) to form triple helical 2'-O-methyl RNA/DNA-hybrid constructs. The potential of the aminoglycoside moieties to stabilize the triple helical constructs were studied by UV-melting profile analysis.
Downloadable publication This is an electronic reprint of the original article. |  |
