Universal platform for quantitative analysis of DNA transposition



: Pajunen MI, Rasila TS, Happonen LJ, Lamberg A, Haapa-Paananen S, Kiljunen S, Savilahti H

Publisher: BIOMED CENTRAL LTD

: 2010

: Mobile DNA

: MOBILE DNA

: MOBILE DNA-UK

: 24

: 1

: 24

: 14

: 1759-8753

DOI: https://doi.org/10.1186/1759-8753-1-24

: http://www.mobilednajournal.com/content/1/1/24


Conclusions: The established universal papillation assay platform should be widely applicable to a variety of mobile elements. It can be used for mechanistic studies to dissect transposition and provides a means to screen or scrutinise transposase mutants and genes encoding host factors. In succession, improved versions of transposition systems should yield better tools for molecular biology and offer versatile genome modification vehicles for many types of studies, including gene therapy and stem cell research.



Last updated on 2024-26-11 at 12:48