A1 Refereed original research article in a scientific journal
Universal platform for quantitative analysis of DNA transposition
Authors: Pajunen MI, Rasila TS, Happonen LJ, Lamberg A, Haapa-Paananen S, Kiljunen S, Savilahti H
Publisher: BIOMED CENTRAL LTD
Publication year: 2010
Journal: Mobile DNA
Journal name in source: MOBILE DNA
Journal acronym: MOBILE DNA-UK
Number in series: 24
Volume: 1
Issue: 24
Number of pages: 14
ISSN: 1759-8753
DOI: https://doi.org/10.1186/1759-8753-1-24(external)
Web address : http://www.mobilednajournal.com/content/1/1/24(external)
Abstract
Conclusions: The established universal papillation assay platform should be widely applicable to a variety of mobile elements. It can be used for mechanistic studies to dissect transposition and provides a means to screen or scrutinise transposase mutants and genes encoding host factors. In succession, improved versions of transposition systems should yield better tools for molecular biology and offer versatile genome modification vehicles for many types of studies, including gene therapy and stem cell research.
Conclusions: The established universal papillation assay platform should be widely applicable to a variety of mobile elements. It can be used for mechanistic studies to dissect transposition and provides a means to screen or scrutinise transposase mutants and genes encoding host factors. In succession, improved versions of transposition systems should yield better tools for molecular biology and offer versatile genome modification vehicles for many types of studies, including gene therapy and stem cell research.
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