Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy
: Tibor Z. Veres, Tamás Kopcsányi, Marko Tirri, Armin Braun, Masayuki Miyasaka, Ronald N. Germain, Sirpa Jalkanen, Marko Salmi
Publisher: NATURE PUBLISHING GROUP
: 2017
: Scientific Reports
: SCIENTIFIC REPORTS
: SCI REP-UK
: 694
: 7
: 9
: 2045-2322
DOI: https://doi.org/10.1038/s41598-017-00769-6
: https://research.utu.fi/converis/portal/detail/Publication/20538454
The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.
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