A1 Refereed original research article in a scientific journal

Intubation-free in vivo imaging of the tracheal mucosa using two-photon microscopy



Authors: Tibor Z. Veres, Tamás Kopcsányi, Marko Tirri, Armin Braun, Masayuki Miyasaka, Ronald N. Germain, Sirpa Jalkanen, Marko Salmi

Publisher: NATURE PUBLISHING GROUP

Publication year: 2017

Journal: Scientific Reports

Journal name in source: SCIENTIFIC REPORTS

Journal acronym: SCI REP-UK

Article number: 694

Volume: 7

Number of pages: 9

ISSN: 2045-2322

DOI: https://doi.org/10.1038/s41598-017-00769-6

Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/20538454


Abstract
The mucosal layer of conducting airways is the primary tissue exposed to inhaled microorganisms, allergens and pollutants. We developed an in vivo two-photon microscopic approach that allows performing dynamic imaging studies in the mouse trachea, which is a commonly used in vivo model of human small-diameter bronchi. By providing stabilized access to the tracheal mucosa without intubation, our setup uniquely allows dynamic in vivo imaging of mucociliary clearance and steady-state immune cell behavior within the complex airway mucosal tissue.

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Last updated on 2024-26-11 at 21:19