Two-photon excitation in fluorescence polarization receptor-ligand binding assay



: Tirri M., Huttunen R., Toivonen J., Härkönen P., Soini J., Hänninen P.

: 2005

 Journal of Biomolecular Screening

: Journal of Biomolecular Screening

: 10

: 4

: 314

: 319

: 6

: 1087-0571

DOI: https://doi.org/10.1177/1087057104273334

: http://api.elsevier.com/content/abstract/scopus_id:20444425639


Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay. © 2005 The Society for Biomolecular Screening.



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