A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Two-photon excitation in fluorescence polarization receptor-ligand binding assay
Tekijät: Tirri M., Huttunen R., Toivonen J., Härkönen P., Soini J., Hänninen P.
Julkaisuvuosi: 2005
Lehti: Journal of Biomolecular Screening
Tietokannassa oleva lehden nimi: Journal of Biomolecular Screening
Vuosikerta: 10
Numero: 4
Aloitussivu: 314
Lopetussivu: 319
Sivujen määrä: 6
ISSN: 1087-0571
DOI: https://doi.org/10.1177/1087057104273334
Verkko-osoite: http://api.elsevier.com/content/abstract/scopus_id:20444425639
Tiivistelmä
Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay. © 2005 The Society for Biomolecular Screening.
Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay. © 2005 The Society for Biomolecular Screening.
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