A1 Refereed original research article in a scientific journal
Two-photon excitation in fluorescence polarization receptor-ligand binding assay
Authors: Tirri M., Huttunen R., Toivonen J., Härkönen P., Soini J., Hänninen P.
Publication year: 2005
Journal:: Journal of Biomolecular Screening
Journal name in source: Journal of Biomolecular Screening
Volume: 10
Issue: 4
First page : 314
Last page: 319
Number of pages: 6
ISSN: 1087-0571
DOI: https://doi.org/10.1177/1087057104273334
Web address : http://api.elsevier.com/content/abstract/scopus_id:20444425639
Abstract
Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay. © 2005 The Society for Biomolecular Screening.
Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay. © 2005 The Society for Biomolecular Screening.
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