Jarmo Niemi
 Ph.D.
| 
 jarnie@utu.fi +358 29 450 4701 +358 50 470 4758 Tykistökatu 6 Turku : B6130 | 
Antibiotic biosynthesis; biology of Streptomyces; protein research
I started work with antibiotic biosynthesis in 1985, when I worked
for the Finnish pharmaceutical company Leiras, and have continued it
since, except for 1995-1998, when I worked in a project concerning
high-throughput screening methods for peptide receptor agonists.
 
My Ph. D. thesis in 1995 was about "Hybrid
Anthracycline Antibiotics: Analysis and
Application
of Rhodomycin Biosynthetic Genes from Streptomyces purpurascens".
My
 research has concerned the biosynthesis of antibiotics by Streptomyces,
 its molecular biology, enzymology and structural biology. I continue to
 participate in the research of the "Antibiotic biosynthesis 
engineering" group http://abelab.utu.fi/
I teach several initial level Biochemistry courses at the University of Turku; BKEM5030 Cell and Biomolecules - Theory, BKEM5010 Microbiology - Theory, BKEM5011 Microbiology - Practice and part of BKEM5160 Cell and Energy - Theory and BKEM5161 Cell and Energy - Practice.
I also teach the course BJ01A1060 Basic Biochemistry at the Lappeenranta University of Technology.
- Crystallization and preliminary X-ray diffraction studies of aclacinomycin-10-methyl esterase and aclacinomycin-10-hydroxylase from Streptomyces purpurascens  (2003)  - Acta Crystallographica Section D: Biological Crystallography
 
- Crystal structure of aclacinomycin-10-hydroxylase, a S-adenosyl-L-methionine-dependent methyltransferase homolog involved in anthracycline biosynthesis in Streptomyces purpurascens  (2003)   Jansson A, Niemi J, Lindqvist Y, Mantsala P, Schneider G
- Crystal structure of aclacinomycin methylesterase with bound product analogues - Implications for anthracycline recognition and mechanism  (2003)  - Journal of Biological Chemistry
 
- Anthracycline biosynthesis: Steps, enzymes and genes  (2002)  Microbial Secondary Metabolites: Biosynthesis, Genetics and Regulation Niemi Jarmo, Kantola Jaana, Ylihonko Kristiina, Mäntsälä Pekka
- Modification of aklavinone and aclacinomycins in vitro and in vivo by rhodomycin biosynthesis gene products  (2002)  - FEMS Microbiology Letters
 
- Modifications of aclacinomycin T by aclacinomycin methyl esterase (RdmC) and aclacinomycin-10-hydroxylase (RdmB) from Streptomyces purpurascens  (2000)  - Biochimica et Biophysica Acta: Protein Structure and Molecular Enzymology
 
- Characterization of aklavinone-11-hydroxylase from Streptomyces purpurascens  (1999)  - Biochimica et Biophysica Acta: Protein Structure and Molecular Enzymology
 
- Hybrid anthracyclines by a genetically engineered Streptomyces galilaeus mutant  (1997)  - Bioorganic and Medicinal Chemistry Letters
 
- NUCLEOTIDE-SEQUENCES AND EXPRESSION OF GENES FROM STREPTOMYCES-PURPURASCENS THAT CAUSE THE PRODUCTION OF NEW ANTHRACYCLINES IN STREPTOMYCES-GALILAEUS  (1995)  - Journal of Bacteriology
 
- HYBRID ANTHRACYCLINE ANTIBIOTICS - PRODUCTION OF NEW ANTHRACYCLINES BY CLONED GENES FROM STREPTOMYCES-PURPURASCENS IN STREPTOMYCES-GALILAEUS  (1994)  - Microbiology
 
- ISOLATION AND CHARACTERIZATION OF ACLACINOMYCIN-A-NON-PRODUCING STREPTOMYCES-GALILAEUS (ATCC-31615) MUTANTS  (1994)  - Microbiology
 
- TRACTOR, a program to locate subclones in a nucleotide sequence using only one sequencing reaction  (1994)  - Computer Applications in the Biosciences
 
 Mäntsälä P
- ABAA, A NEW PLEIOTROPIC REGULATORY LOCUS FOR ANTIBIOTIC PRODUCTION IN STREPTOMYCES-COELICOLOR  (1992)  - Journal of Bacteriology
 
- CLONING AND EXPRESSION IN A HETEROLOGOUS HOST OF THE COMPLETE SET OF GENES FOR BIOSYNTHESIS OF THE STREPTOMYCES-COELICOLOR ANTIBIOTIC UNDECYLPRODIGIOSIN  (1990)  - Gene
 
- EXPRESSION OF HETEROLOGOUS GENE AND SECRETION OF ITS PRODUCT IN ESCHERICHIA-COLI  (1987)  - Protein Engineering
 
- A NEW APPROACH TO HIGH-SENSITIVITY DIFFERENTIAL HYBRIDIZATION  (1986)  - Gene
 





