A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis
Tekijät: Moreno-Layseca Paulina, Jäntti Niklas Z, Godbole Rashmi, Sommer Christian, Jacquemet Guillaume, Al-Akhrass Hussein, Conway James RW, Kronqvist Pauliina, Kallionpää Roosa E, Oliveira-Ferrer Leticia, Cervero Pasquale, Linder Stefan, Aepfelbacher Martin, Zauber Henrik, Rae James, Parton Robert G, Disanza Andrea, Scita Giorgio, Mayor Satyajit, Selbach Matthias, Veltel Stefan, Ivaska Johanna
Kustantaja: NATURE PORTFOLIO
Julkaisuvuosi: 2021
Journal: Nature Cell Biology
Tietokannassa oleva lehden nimi: NATURE CELL BIOLOGY
Lehden akronyymi: NAT CELL BIOL
Vuosikerta: 23
Numero: 10
Aloitussivu: 1073
Lopetussivu: 1084
Sivujen määrä: 40
ISSN: 1465-7392
eISSN: 1476-4679
DOI: https://doi.org/10.1038/s41556-021-00767-x
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/67555401
Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery-Arf1, IRSp53 and actin-and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route.Moreno-Layseca et al. identify Swip1 as an integrin-specific endocytic adaptor controlling the dynamics of integrin adhesion complexes as well as the migration and invasion of breast cancer cells.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
