A1 Refereed original research article in a scientific journal
Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis
Authors: Moreno-Layseca Paulina, Jäntti Niklas Z, Godbole Rashmi, Sommer Christian, Jacquemet Guillaume, Al-Akhrass Hussein, Conway James RW, Kronqvist Pauliina, Kallionpää Roosa E, Oliveira-Ferrer Leticia, Cervero Pasquale, Linder Stefan, Aepfelbacher Martin, Zauber Henrik, Rae James, Parton Robert G, Disanza Andrea, Scita Giorgio, Mayor Satyajit, Selbach Matthias, Veltel Stefan, Ivaska Johanna
Publisher: NATURE PORTFOLIO
Publication year: 2021
Journal: Nature Cell Biology
Journal name in source: NATURE CELL BIOLOGY
Journal acronym: NAT CELL BIOL
Volume: 23
Issue: 10
First page : 1073
Last page: 1084
Number of pages: 40
ISSN: 1465-7392
eISSN: 1476-4679
DOI: https://doi.org/10.1038/s41556-021-00767-x
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/67555401
Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and lipids. While the core molecular players of CG-endocytosis have been recently defined, evidence of cargo-specific adaptors or selective uptake of proteins for the pathway are lacking. Here we identify the actin-binding protein Swiprosin-1 (Swip1, EFHD2) as a cargo-specific adaptor for CG-endocytosis. Swip1 couples active Rab21-associated integrins with key components of the CG-endocytic machinery-Arf1, IRSp53 and actin-and is critical for integrin endocytosis. Through this function, Swip1 supports integrin-dependent cancer-cell migration and invasion, and is a negative prognostic marker in breast cancer. Our results demonstrate a previously unknown cargo selectivity for the CG pathway and a role for specific adaptors in recruitment into this endocytic route.Moreno-Layseca et al. identify Swip1 as an integrin-specific endocytic adaptor controlling the dynamics of integrin adhesion complexes as well as the migration and invasion of breast cancer cells.
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