A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Detection of bladder cancer with aberrantly fucosylated ITGA3
Tekijät: Islam Md Khirul, Syed Parvez, Dhondt Bert, Gidwani Kamlesh, Pettersson Kim, Lamminmäki Urpo, Leivo Janne
Kustantaja: Academic Press Inc Elsevier Science
Julkaisuvuosi: 2021
Journal: Analytical Biochemistry
Tietokannassa oleva lehden nimi: ANALYTICAL BIOCHEMISTRY
Lehden akronyymi: ANAL BIOCHEM
Artikkelin numero: ARTN 114283
Vuosikerta: 628
Sivujen määrä: 5
ISSN: 0003-2697
eISSN: 1096-0309
DOI: https://doi.org/10.1016/j.ab.2021.114283
Verkko-osoite: https://www.sciencedirect.com/science/article/pii/S0003269721001846?via%3Dihub
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/66651036
We describe a simple, non-invasive assay to identify fucosylated-glycoisoform of integrin alpha-3 (ITGA3) directly from unprocessed urine. ITGA3 was detected directly from the urine of bladder cancer (BlCa) (n = 13) and benign prostatic hyperplasia (BPH) (n = 9) patients with the use of lectins coated on europium-dopednanoparticles (Eu3+-NPs). Lectin Ulex europaeus agglutinin-I (UEA) showed enhanced binding with BlCaderived ITGA3. The evaluation with individual samples showed that a glycovariant ITGA3-UEA assay could significantly discriminate BlCa from BPH patients (p = 0.007). The detection of aberrantly fucosylated-isoform of ITGA3 from urine can be used to distinguish BlCa from age-matched benign controls in a simple sandwich assay.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
