A1 Refereed original research article in a scientific journal
Detection of bladder cancer with aberrantly fucosylated ITGA3
Authors: Islam Md Khirul, Syed Parvez, Dhondt Bert, Gidwani Kamlesh, Pettersson Kim, Lamminmäki Urpo, Leivo Janne
Publisher: Academic Press Inc Elsevier Science
Publication year: 2021
Journal: Analytical Biochemistry
Journal name in source: ANALYTICAL BIOCHEMISTRY
Journal acronym: ANAL BIOCHEM
Article number: ARTN 114283
Volume: 628
Number of pages: 5
ISSN: 0003-2697
eISSN: 1096-0309
DOI: https://doi.org/10.1016/j.ab.2021.114283
Web address : https://www.sciencedirect.com/science/article/pii/S0003269721001846?via%3Dihub
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/66651036
We describe a simple, non-invasive assay to identify fucosylated-glycoisoform of integrin alpha-3 (ITGA3) directly from unprocessed urine. ITGA3 was detected directly from the urine of bladder cancer (BlCa) (n = 13) and benign prostatic hyperplasia (BPH) (n = 9) patients with the use of lectins coated on europium-dopednanoparticles (Eu3+-NPs). Lectin Ulex europaeus agglutinin-I (UEA) showed enhanced binding with BlCaderived ITGA3. The evaluation with individual samples showed that a glycovariant ITGA3-UEA assay could significantly discriminate BlCa from BPH patients (p = 0.007). The detection of aberrantly fucosylated-isoform of ITGA3 from urine can be used to distinguish BlCa from age-matched benign controls in a simple sandwich assay.
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