A1 Journal article – refereed

Detection of bladder cancer with aberrantly fucosylated ITGA3




List of Authors: Islam Md Khirul, Syed Parvez, Dhondt Bert, Gidwani Kamlesh, Pettersson Kim, Lamminmäki Urpo, Leivo Janne

Publisher: Academic Press Inc Elsevier Science

Publication year: 2021

Journal: Analytical Biochemistry

Journal name in source: ANALYTICAL BIOCHEMISTRY

Journal acronym: ANAL BIOCHEM

Volume number: 628

Number of pages: 5

ISSN: 0003-2697

eISSN: 1096-0309

DOI: http://dx.doi.org/10.1016/j.ab.2021.114283

URL: https://www.sciencedirect.com/science/article/pii/S0003269721001846?via%3Dihub


Abstract

We describe a simple, non-invasive assay to identify fucosylated-glycoisoform of integrin alpha-3 (ITGA3) directly from unprocessed urine. ITGA3 was detected directly from the urine of bladder cancer (BlCa) (n = 13) and benign prostatic hyperplasia (BPH) (n = 9) patients with the use of lectins coated on europium-dopednanoparticles (Eu3+-NPs). Lectin Ulex europaeus agglutinin-I (UEA) showed enhanced binding with BlCaderived ITGA3. The evaluation with individual samples showed that a glycovariant ITGA3-UEA assay could significantly discriminate BlCa from BPH patients (p = 0.007). The detection of aberrantly fucosylated-isoform of ITGA3 from urine can be used to distinguish BlCa from age-matched benign controls in a simple sandwich assay.


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Last updated on 2021-07-09 at 14:44