The effect of phytic acid on enzymatic degradation of dentin



: Forgione Diletta, Nassar Mohannad, Seseogullari-Dirihan Roda, Thitthaweerat Suppason, Tezvergil-Mutluay Arzu

Publisher: WILEY

: 2021

: European Journal of Oral Sciences

: EUROPEAN JOURNAL OF ORAL SCIENCES

: EUR J ORAL SCI

: e12771

: 129

: 2

: 8

: 0909-8836

: 1600-0722

DOI: https://doi.org/10.1111/eos.12771(external)

: https://onlinelibrary.wiley.com/doi/10.1111/eos.12771(external)

: https://research.utu.fi/converis/portal/detail/Publication/53984874(external)


We evaluated the effect of phytic acid on matrix metalloproteinase (MMP)- or cysteine cathepsin (CC)-mediated dentin degradation. Demineralized dentin beams were divided into five groups (n = 12) and treated with 1%, 2%, or 3% phytic acid or with 37% phosphoric acid. Untreated demineralized beams served as controls. After incubation for 1 or 3 wk, dry mass loss was determined and aliquots of incubation media were analysed for cross-linked telopeptide of type I collagen (ICTP) fragments for MMP-mediated and c-terminal telopeptide of type I collagen (CTX) for cathepsin-k-mediated degradation. The direct effect of phytic acid was evaluated using MMP activity assay. Data were analysed using repeated-measures anova. ICTP releases with 1% and 2% phytic acid treatment were statistically significantly lower than those following phosphoric acid treatment at 3 wk. The CTX release for phytic acid-treated beams at 3 wk was not significantly different from that of untreated control beams, but it was significantly lower than that of phosphoric acid-treated beams. Their MMP activities at 3 wk were not significantly different from those of the controls but they were significantly lower than those seen for phosphoric acid-treated beams. Compared to phosphoric acid, phytic acid treatment resulted in a reduced dentinal host-derived endogenous enzymatic activity and collagen degradation.

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