A1 Refereed original research article in a scientific journal
The effect of phytic acid on enzymatic degradation of dentin
Authors: Forgione Diletta, Nassar Mohannad, Seseogullari-Dirihan Roda, Thitthaweerat Suppason, Tezvergil-Mutluay Arzu
Publisher: WILEY
Publication year: 2021
Journal: European Journal of Oral Sciences
Journal name in source: EUROPEAN JOURNAL OF ORAL SCIENCES
Journal acronym: EUR J ORAL SCI
Article number: e12771
Volume: 129
Issue: 2
Number of pages: 8
ISSN: 0909-8836
eISSN: 1600-0722
DOI: https://doi.org/10.1111/eos.12771(external)
Web address : https://onlinelibrary.wiley.com/doi/10.1111/eos.12771(external)
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/53984874(external)
We evaluated the effect of phytic acid on matrix metalloproteinase (MMP)- or cysteine cathepsin (CC)-mediated dentin degradation. Demineralized dentin beams were divided into five groups (n = 12) and treated with 1%, 2%, or 3% phytic acid or with 37% phosphoric acid. Untreated demineralized beams served as controls. After incubation for 1 or 3 wk, dry mass loss was determined and aliquots of incubation media were analysed for cross-linked telopeptide of type I collagen (ICTP) fragments for MMP-mediated and c-terminal telopeptide of type I collagen (CTX) for cathepsin-k-mediated degradation. The direct effect of phytic acid was evaluated using MMP activity assay. Data were analysed using repeated-measures anova. ICTP releases with 1% and 2% phytic acid treatment were statistically significantly lower than those following phosphoric acid treatment at 3 wk. The CTX release for phytic acid-treated beams at 3 wk was not significantly different from that of untreated control beams, but it was significantly lower than that of phosphoric acid-treated beams. Their MMP activities at 3 wk were not significantly different from those of the controls but they were significantly lower than those seen for phosphoric acid-treated beams. Compared to phosphoric acid, phytic acid treatment resulted in a reduced dentinal host-derived endogenous enzymatic activity and collagen degradation.
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