A1 Refereed original research article in a scientific journal

The diatom test for drowning: an unreported source of diatom contamination




AuthorsLunetta, Philippe; Virri, Johanna; Weckström, Jan

PublisherSpringer Science and Business Media LLC

Publishing placeNEW YORK

Publication year2025

JournalInternational Journal of Legal Medicine

Journal name in sourceInternational Journal of Legal Medicine

Journal acronymINT J LEGAL MED

Number of pages4

ISSN0937-9827

eISSN1437-1596

DOIhttps://doi.org/10.1007/s00414-025-03527-w

Web address https://doi.org/10.1007/s00414-025-03527-w

Self-archived copy’s web addresshttps://research.utu.fi/converis/portal/detail/Publication/499585964


Abstract
We describe here a previously unreported source of diatom contamination that may prove relevant in routine diatom analysis in suspected drowning cases. Glass microscope slides utilized in the process, during their manufacture, undergo a washing process, after which, diatomaceous earth (kieselguhr, fossil diatoms) is added to avoid producing stuck slides during the last rinse. The diatomaceous earth can be removed at this point only by means of aggressive cleaning, but the possibility exists that some fossil diatoms will remain on the slides. This potential contamination source explained a recurrent and otherwise puzzling appearance of Aulacoseira diatom genus in samples both from definite drowning cases and from non-drowned control cases. Discrimination by expert diatomologists between present-day fresh diatoms and fossil diatoms, together with awareness of the ubiquity of diatoms in nature and of their extensive use in the manufacturing industry, corroborate our view that potential sources of laboratory contamination should not be a rational impediment to performing diatom tests.

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Funding information in the publication
Open Access funding provided by University of Turku (including Turku University Central Hospital). Open access funding provided by University of Turku (UTU) including Turku University Central Hospital.


Last updated on 2025-15-09 at 13:36