A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Integration of GC-MS, ROAV, and chemometrics to characterize key differential volatile compounds in wild and cultivated blueberries from Northeast China
Tekijät: Xie, Xu; Nie, Yujie; Wang, Yuehua; Wen, Bosu; Shang, Junzhe; Guo, Xin; Tian, Jinlong; Wang, Yumeng; Zhang, Yiming; Li, Siyuan; Li, Yue; Hu, Bowen; Yang, Baoru; Zhou, Ying; Wang, Mingqian; Li, Bin
Kustantaja: Elsevier BV
Kustannuspaikka: AMSTERDAM
Julkaisuvuosi: 2025
Journal: LWT - Food Science and Technology
Tietokannassa oleva lehden nimi: LWT
Lehden akronyymi: LWT-FOOD SCI TECHNOL
Artikkelin numero: 117950
Vuosikerta: 225
Sivujen määrä: 12
ISSN: 0023-6438
eISSN: 1096-1127
DOI: https://doi.org/10.1016/j.lwt.2025.117950
Verkko-osoite: https://doi.org/10.1016/j.lwt.2025.117950
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/498988441
The flavor of blueberries influences consumers' purchasing behavior and satisfaction, holding significant research value for enhancing market competitiveness. In this study, we analyzed the volatile metabolites of Wild blueberry (Wb) and Cultivated blueberry (Cb) in Northeast China (NC) using headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS), identifying 618 volatile organic compounds (VOCs). Through multivariate statistical analysis, 140 volatile differential compounds were detected between Wb and Cb, including trans-alpha-Bergamotene; 2-ButenoicAcid,2-methyl-; Benzoic acid,2-hydroxy-, ethylester; Benzoic acid, ethyl ester, above 4 VOCs were unique to Wb; while 28 VOCs, such as Carotol and 1Hexanol, were unique to Cb. KEGG enrichment analysis indicated that alpha-Linolenic acid metabolism and Inflammatory mediator regulation of TRP channels are the key pathways for volatile differential substance formation. By employing relative odor activity value (ROAV) and chemometrics, 131 key aroma-presenting substances were identified. Among them, beta-Ionone, 2(5H)-Furanone, 5-ethyl-3-hydroxy-4-methyl- with sweet and fruity aroma were the most crucial. Following comprehensive, in-depth analysis by HS-SPME-GC-MS and ROAV, Carotol and 1-Hexanol were found to be useful as biomarker compounds for distinguishing Wb and Cb. This study provides a theoretical basis for distinguishing the aroma characteristics of Wb and Cb in NC, the processing adaptability of different varieties of blueberries.
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This work was supported by the National Natural Science Foundation of China (NO.32472368) , and the National Key R & D Program of China (2022YFD2100803-03) .
