A1 Refereed original research article in a scientific journal
Integration of GC-MS, ROAV, and chemometrics to characterize key differential volatile compounds in wild and cultivated blueberries from Northeast China
Authors: Xie, Xu; Nie, Yujie; Wang, Yuehua; Wen, Bosu; Shang, Junzhe; Guo, Xin; Tian, Jinlong; Wang, Yumeng; Zhang, Yiming; Li, Siyuan; Li, Yue; Hu, Bowen; Yang, Baoru; Zhou, Ying; Wang, Mingqian; Li, Bin
Publisher: Elsevier BV
Publishing place: AMSTERDAM
Publication year: 2025
Journal: LWT - Food Science and Technology
Journal name in source: LWT
Journal acronym: LWT-FOOD SCI TECHNOL
Article number: 117950
Volume: 225
Number of pages: 12
ISSN: 0023-6438
eISSN: 1096-1127
DOI: https://doi.org/10.1016/j.lwt.2025.117950
Web address : https://doi.org/10.1016/j.lwt.2025.117950
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/498988441
The flavor of blueberries influences consumers' purchasing behavior and satisfaction, holding significant research value for enhancing market competitiveness. In this study, we analyzed the volatile metabolites of Wild blueberry (Wb) and Cultivated blueberry (Cb) in Northeast China (NC) using headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS), identifying 618 volatile organic compounds (VOCs). Through multivariate statistical analysis, 140 volatile differential compounds were detected between Wb and Cb, including trans-alpha-Bergamotene; 2-ButenoicAcid,2-methyl-; Benzoic acid,2-hydroxy-, ethylester; Benzoic acid, ethyl ester, above 4 VOCs were unique to Wb; while 28 VOCs, such as Carotol and 1Hexanol, were unique to Cb. KEGG enrichment analysis indicated that alpha-Linolenic acid metabolism and Inflammatory mediator regulation of TRP channels are the key pathways for volatile differential substance formation. By employing relative odor activity value (ROAV) and chemometrics, 131 key aroma-presenting substances were identified. Among them, beta-Ionone, 2(5H)-Furanone, 5-ethyl-3-hydroxy-4-methyl- with sweet and fruity aroma were the most crucial. Following comprehensive, in-depth analysis by HS-SPME-GC-MS and ROAV, Carotol and 1-Hexanol were found to be useful as biomarker compounds for distinguishing Wb and Cb. This study provides a theoretical basis for distinguishing the aroma characteristics of Wb and Cb in NC, the processing adaptability of different varieties of blueberries.
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Funding information in the publication:
This work was supported by the National Natural Science Foundation of China (NO.32472368) , and the National Key R & D Program of China (2022YFD2100803-03) .
