A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
High-resolution live imaging of tardigrade response to anoxia
Tekijät: Haapanen-Saaristo, Anna-Mari; Calhim, Sara; Paatero, Ilkka
Kustantaja: Elsevier BV
Kustannuspaikka: OXFORD
Julkaisuvuosi: 2025
Journal: Micron
Tietokannassa oleva lehden nimi: Micron
Lehden akronyymi: MICRON
Artikkelin numero: 103847
Vuosikerta: 196-197
Sivujen määrä: 10
ISSN: 0968-4328
eISSN: 1878-4291
DOI: https://doi.org/10.1016/j.micron.2025.103847
Verkko-osoite: https://doi.org/10.1016/j.micron.2025.103847
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/498615986
Tardigrades are well-known for their ability to tolerate extreme environmental conditions such as heat, drought and lack of oxygen by undergoing cryptobiosis. The molecular responses to stress have been studied in detail, but the physiological and morphogenetic changes during cryptobiosis are less understood. We developed new live high-resolution fluorescence microscopy protocols to visualize the tardigrade response to lack of oxygen-anoxybiosis. High-resolution time-lapse imaging enabled analysis of cellular morphology and tracking of cell movements during anoxybiosis. These analyses revealed considerable changes in morphology, composition and movement of storage cells. Our observations and new imaging protocols can be used to study morphological and cellular response to stress in tardigrades.
Ladattava julkaisu This is an electronic reprint of the original article. |
Julkaisussa olevat rahoitustiedot:
University of Turku (I.P.), BusinessFinland (1718/31/2021), Finnish Foundation for Cardiovascular Research (230066).