A1 Refereed original research article in a scientific journal
High-resolution live imaging of tardigrade response to anoxia
Authors: Haapanen-Saaristo, Anna-Mari; Calhim, Sara; Paatero, Ilkka
Publisher: Elsevier BV
Publishing place: OXFORD
Publication year: 2025
Journal: Micron
Journal name in source: Micron
Journal acronym: MICRON
Article number: 103847
Volume: 196-197
Number of pages: 10
ISSN: 0968-4328
eISSN: 1878-4291
DOI: https://doi.org/10.1016/j.micron.2025.103847
Web address : https://doi.org/10.1016/j.micron.2025.103847
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/498615986
Tardigrades are well-known for their ability to tolerate extreme environmental conditions such as heat, drought and lack of oxygen by undergoing cryptobiosis. The molecular responses to stress have been studied in detail, but the physiological and morphogenetic changes during cryptobiosis are less understood. We developed new live high-resolution fluorescence microscopy protocols to visualize the tardigrade response to lack of oxygen-anoxybiosis. High-resolution time-lapse imaging enabled analysis of cellular morphology and tracking of cell movements during anoxybiosis. These analyses revealed considerable changes in morphology, composition and movement of storage cells. Our observations and new imaging protocols can be used to study morphological and cellular response to stress in tardigrades.
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Funding information in the publication:
University of Turku (I.P.), BusinessFinland (1718/31/2021), Finnish Foundation for Cardiovascular Research (230066).
