A Diagnostic Approach to Separate Acute Human Bocavirus 1 Respiratory Tract Infection From Long-lasting Virus Shedding - UTU Research Portal

A1 Refereed original research article in a scientific journal

A Diagnostic Approach to Separate Acute Human Bocavirus 1 Respiratory Tract Infection From Long-lasting Virus Shedding

Authors: Thapa, Rajita Rayamajhi; Nascimento-Carvalho, Cristiana; Allander, Tobias; Jartti, Tuomas; Söderlund-Venermo, Maria

Publisher: Oxford University Press (OUP)

Publishing place: CARY

Publication year: 2025

Journal: Journal of Infectious Diseases

Journal name in source: The Journal of Infectious Diseases

Journal acronym: J INFECT DIS

Number of pages: 5

ISSN: 0022-1899

eISSN: 1537-6613

DOI: https://doi.org/10.1093/infdis/jiaf130

Web address : https://doi.org/10.1093/infdis/jiaf130

Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/491603217

Abstract

Human bocavirus 1 (HBoV1) causes mild to life-threatening respiratory tract infections in children but may persist in the airways for months. Currently used polymerase chain reaction may thus lead to false diagnoses and irrelevant codetections. Our aim was to differentiate acute infections from persistent shedding by pretreatment of airway or serum samples with an endonuclease, followed by polymerase chain reaction (ePCR). We show that HBoV1 DNA is protected by a capsid in the acute phase but not in persistent shedding, and we provide proof of concept of a novel test that may be applied in routine diagnosis of acute HBoV1 respiratory tract infection for more accurate results.We show that human bocavirus 1 DNA is encapsidated in the acute phase but not in persistent shedding, and we introduce a novel test-endonuclease polymerase chain reaction-that can distinguish between these scenarios for a more accurate diagnosis of acute human bocavirus 1 respiratory tract infection.

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Funding information in the publication:
This work was supported by the Doctoral Programme in Population Health (to R. R. T.); the Sigrid Jusélius Foundation (M. S.-V.); and the Life and Health Medical Association (M. S.-V.).