A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
A Diagnostic Approach to Separate Acute Human Bocavirus 1 Respiratory Tract Infection From Long-lasting Virus Shedding
Tekijät: Thapa, Rajita Rayamajhi; Nascimento-Carvalho, Cristiana; Allander, Tobias; Jartti, Tuomas; Söderlund-Venermo, Maria
Kustantaja: Oxford University Press (OUP)
Kustannuspaikka: CARY
Julkaisuvuosi: 2025
Journal: Journal of Infectious Diseases
Tietokannassa oleva lehden nimi: The Journal of Infectious Diseases
Lehden akronyymi: J INFECT DIS
Sivujen määrä: 5
ISSN: 0022-1899
eISSN: 1537-6613
DOI: https://doi.org/10.1093/infdis/jiaf130
Verkko-osoite: https://doi.org/10.1093/infdis/jiaf130
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/491603217
Human bocavirus 1 (HBoV1) causes mild to life-threatening respiratory tract infections in children but may persist in the airways for months. Currently used polymerase chain reaction may thus lead to false diagnoses and irrelevant codetections. Our aim was to differentiate acute infections from persistent shedding by pretreatment of airway or serum samples with an endonuclease, followed by polymerase chain reaction (ePCR). We show that HBoV1 DNA is protected by a capsid in the acute phase but not in persistent shedding, and we provide proof of concept of a novel test that may be applied in routine diagnosis of acute HBoV1 respiratory tract infection for more accurate results.We show that human bocavirus 1 DNA is encapsidated in the acute phase but not in persistent shedding, and we introduce a novel test-endonuclease polymerase chain reaction-that can distinguish between these scenarios for a more accurate diagnosis of acute human bocavirus 1 respiratory tract infection.
Ladattava julkaisu This is an electronic reprint of the original article. |  |
Julkaisussa olevat rahoitustiedot:
This work was supported by the Doctoral Programme in Population Health (to R. R. T.); the Sigrid Jusélius Foundation (M. S.-V.); and the Life and Health Medical Association (M. S.-V.).
